Hypochlorous acid induced structural and conformational modifications in human DNA: A multi-spectroscopic study

Tantry, I.Q. and Waris, S. and Habib, S. and Khan, R.H. and Mahmood, R. and Ali, A. (2018) Hypochlorous acid induced structural and conformational modifications in human DNA: A multi-spectroscopic study. International Journal of Biological Macromolecules, 106. pp. 551-558. ISSN 1418130

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Abstract

Hypochlorous acid (HOCl) is generated by activated phagocytes at the site of inflammation. Exposure of DNA to HOCl results in base and nucleotide modifications causing DNA damage, which is one of the leading causes of various pathological conditions including carcinogenesis. In the present work, various biophysical techniques were used to study HOCl induced structural and conformational changes in human placental DNA. The HOCl modified DNA showed hyperchromicity, reduced fluorescence and decrease in melting temperature. Circular dichorism (CD) and Fourier transform infra-red (FT-IR) studies exhibited conformational changes and shift in band positions of DNA, respectively, suggesting structural changes. Agarose gel electrophoresis and scanning electron microscopy showed strand breakage and decreased aggregation. These results suggest that HOCl causes conformational and structural perturbations in mammalian DNA, which may consequentially lead to DNA mutations resulting in perturbation of epigenetic signals leading to cancer and autoimmune diseases. © 2017 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: Agarose gel electrophoresis; CD; FT-IR; HOCl; Human placental DNA; SEM; UV spectroscopy
Subjects: R Medicine > R Medicine (General)
Divisions: Faculties > Faculty of Life Sciences > Department of Biochemistry
Depositing User: AMU Library
Date Deposited: 17 Jan 2018 07:57
Last Modified: 17 Jan 2018 09:13
URI: http://ir.amu.ac.in/id/eprint/10772

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