Biophysical insight into the interaction mechanism of plant derived polyphenolic compound tannic acid with homologous mammalian serum albumins

Ishtikhar, M and Ahmad, E. and Siddiqui, Z. and Ahmad, S. and Khan, M.V. and Zaman, M and Siddiqi, M.K and Nusrat, S and Chandel, T.I. and Ajmal., M.R and Khan, R.H. (2018) Biophysical insight into the interaction mechanism of plant derived polyphenolic compound tannic acid with homologous mammalian serum albumins. International Journal of Biological Macromolecules. pp. 2450-2464. ISSN 1418130

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Abstract

Numerous phenolic compounds have been reported in the last decade that have a good antioxidant property and interaction affinity towards mammalian serum albumins. In the present study, we have utilized mammalian serum albumins as a model protein to examine their comparative interaction property with polyphenolic compound tannic acid (TA) by using various spectroscopic and calorimetric methods We have also monitored the esterase and antioxidant activity of mammalian serum albumins in the absence and presence of TA. The obtain results recommended that the TA have a good binding affinity (∼104 to 106 M−1) towards mammalian serum albumins and shows double sequential binding sites, which depends on the concentration of TA that induced the conformational alteration which responsible for the thermal stability of proteins. Binding affinity, structural transition and thermodynamic parameters were calculated from spectroscopic and calorimetric method reveals that non-covalent interaction causes partial conformational alteration in the secondary structure of protein ie.; increase in α-helical content with decrease in β-sheet, random coil and other structure. Meanwhile, we have found that esterase activities of serum albumins were also stabilized against hydrolysis and shows higher antioxidant activity in the presence of TA because albumins its self have an immense antioxidant activity beside TA. © 2017 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: Antioxidant activity; Binding; Esterase activity; Fluorescence spectroscopy; Isothermal titration calorimetry; Polyphenols; Serum albumin; Tannic acid
Divisions: Centres > Interdisciplinary Biotechnology Unit
Depositing User: AMU Library
Date Deposited: 18 Jan 2018 07:13
Last Modified: 11 Feb 2018 13:02
URI: http://ir.amu.ac.in/id/eprint/10777

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