Synthesis, characterization and in vitro screening of a nano-hydroxyapatite/chitosan/ Euryale ferox nanoensemble-an inimitable approach for bone tissue engineering

Shakir, M. and Mirza, S. and Jolly, R. and Rauf, A. and Owais, M. (2018) Synthesis, characterization and in vitro screening of a nano-hydroxyapatite/chitosan/ Euryale ferox nanoensemble-an inimitable approach for bone tissue engineering. New Journal of Chemistry, 42 (1). pp. 363-371. ISSN 11440546

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Abstract

In order to explore novel synthetic bone scaffolds as part of our ongoing research in the field of bone tissue engineering, we have synthesized a biomimmetic, osteoinductive, tricomposite scaffold incorporating Euryale ferox (EF) with nano-hydroxyapatite and chitosan. The surface morphology of the resultant nanocomposite was visualized via SEM and TEM studies which exhibited a rougher surface of n-HA/CS/EF compared to the n-HA/CS nanocomposite with the particle size ranging between 15 and 20 nm. The n-HA/CS/EF scaffold was degraded up to (16.9 ± 0.70)% in contrast to degradation of (7.4 ± 0.2)% for n-HA/CS. The in vitro SBF study exhibited excellent biomineralization capacity for n-HA/CS/EF which was further elucidated using the ARS staining procedure. The MTT assay illustrated significantly better cell viability compared to the n-HA/CS nanocomposite. A comparatively better antibacterial activity of n-HA/CS/EF was observed against a number of bacterial pathogens. The osteogenic differentiation test (ALP assay) assessed the osteoconductivity of the n-HA/CS/EF nanocomposite. Thus, the synthesized scaffold can be envisioned for potential use in reparation of bone defects. © 2018 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Faculties > Faculty of Science > Department of Chemistry
Depositing User: AMU Library
Date Deposited: 19 Jan 2018 04:12
Last Modified: 19 Jan 2018 04:12
URI: http://ir.amu.ac.in/id/eprint/10822

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