Flavonoids-induced redox cycling of copper ions leads to generation of reactive oxygen species: A potential role in cancer chemoprevention

Arif, H. and Sohail, A. and Farhan, M. and Rehman, A.A. and Ahmad, A. and Hadi, S.M. (2018) Flavonoids-induced redox cycling of copper ions leads to generation of reactive oxygen species: A potential role in cancer chemoprevention. International Journal of Biological Macromolecules, 106. pp. 569-578. ISSN 1418130

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Abstract

Flavonoids, a class of polyphenols are known to be effective inducers of apoptosis and cytotoxicity in cancer cells. It is believed that antioxidant activity of polyphenols cannot fully account for induction of apoptosis and chemotherapeutic prevention in various cancers. In this article, by employing single cell alkaline gel electrophoresis (comet assay), we established that antioxidants, flavonoids such as (myricetin = MN, fisetin = FN, quercetin = QN, kaempferol = KL and galangin = GN) can cause cellular DNA breakage, also act as pro-oxidant in presence of transition metal ion such as copper. It was observed that the extent of cellular DNA breakage was found significantly higher in presence of copper. Hydroxyl radicals are generated as a sign of flavonoids’ pro-oxidant nature through redox recycling of copper ions. Further, a dose-dependent inhibition of proliferation of breast cancer cells MDA-MB-231 by MN was found leading to pro-oxidant cell death, as assessed by MTT assay. Since levels of copper are considerably elevated in tissue, cell and serum during various malignancies, suggesting that cancer cells would be more subject to copper induced oxidative DNA breakage. Such a copper dependent pro-oxidant cytotoxic mechanism better explains the anticancer activity and preferential cytotoxicity of dietary phytochemicals against cancer cells. © 2017 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: Comet assay; Copper; Flavonoids; MTT assay; Myricetin; Pro-oxidant; Reactive oxygen species
Divisions: Faculties > Faculty of Life Sciences > Department of Biochemistry
Depositing User: AMU Library
Date Deposited: 20 Jan 2018 04:10
Last Modified: 20 Jan 2018 04:10
URI: http://ir.amu.ac.in/id/eprint/10846

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