Characterization of colchicine binding with normal and glycated albumin: In vitro and molecular docking analysis

Rabbani, N. and Tabrez, S. and Islam, B.U. and Rehman, M.T and Alsenaidy, A.M. and AlAjmi, M.F. and Khan, R.A. and Alsenaidy, M.A. and Khan, M.S. (2017) Characterization of colchicine binding with normal and glycated albumin: In vitro and molecular docking analysis. Journal of Biomolecular Structure and Dynamics. pp. 1-10. ISSN 7391102

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The transport of more than 90% of the drugs viz. anticoagulants, analgesics, and general anesthetics in the blood takes place by albumin. Hence, albumin is the prime protein needs to be investigated to find out the nature of drug binding. Serum albumin molecules are prone to glycation at elevated blood glucose levels as observed in diabetics. In this piece of work, glycation of bovine serum albumin (BSA) was carried out with glyceraldehyde and characterized by molecular docking and fluorometry techniques. Glycation of BSA showed 25% loss of free amino groups and decreased protein fluorescence (60%) with blue shift of 6 nm. The present study was also designed to evaluate the binding of colchicine (an anti-inflammatory drug) to native and glycated BSA and its ability to displace 8-analino-1-nephthalene sulfonic acid (ANS), from the BSA–ANS complex. Binding of ANS to BSA showed strong binding (Ka = 4.4 μM) with native conformation in comparison to glycated state (Ka = 8.4 μM). On the other hand, colchicine was able to quench the fluorescence of native BSA better than glycated BSA and also showed weaker affinity (Ka = 23 μM) for glycated albumin compared with native state (Ka = 16 μM). Molecular docking study showed that both glyceraldehyde and colchicine bind to common residues located near Sudlow’s site I that explain the lower binding of colchicine in the glycated BSA. Based on our results, we believe that reduced drugs-binding affinity to glycated albumin may lead to drugs accumulation and precipitation in diabetic patients.

Item Type: Article
Uncontrolled Keywords: bovine serum albumin; colchicine; drug-binding capability; glycation; glyceraldehyde
Subjects: R Medicine > R Medicine (General)
Divisions: Faculties > Faculty of Life Sciences > Department of Biochemistry
Depositing User: AMU Library
Date Deposited: 29 Jan 2018 07:08
Last Modified: 01 Feb 2018 04:51

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