Partial purification and characterization of glutathione S-transferase from the somatic tissue of Gastrothylax crumenifer (Trematoda: Digenea)

Ahmed, S. and Sohail, A. and Khatoon ., S and Khan, S. and Saifullah, M.K. (2017) Partial purification and characterization of glutathione S-transferase from the somatic tissue of Gastrothylax crumenifer (Trematoda: Digenea). Veterinary World, 10 (12). p. 1493. ISSN 9728988

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Official URL: http://www.veterinaryworld.org/Vol.10/December-201...

Abstract

Aim: Aim of the present study was to carry out the partial purification and biochemical characterization of glutathione S-transferase (GST) from the somatic tissue of ruminal amphistome parasite, Gastrothylax crumenifer (Gc) infecting Indian water buffalo (Bubalus bubalis). Materials and Methods: The crude somatic homogenate of Gc was subjected to progressive ammonium sulfate precipitation followed by size exclusion chromatography in a Sephacryl S 100-HR column. The partially purified GST was assayed spectrophotometrically, and the corresponding enzyme activity was also recorded in polyacrylamide gel. GST isolated from the amphistome parasite was also exposed to variable changes in temperature and the pH gradient of the assay mixture. Results: The precipitated amphistome GST molecules showed maximum activity in the sixth elution fraction. The GST subunit appeared as a single band in the reducing polyacrylamide gel electrophoresis with an apparent molecular weight of 26 kDa. The GST proteins were found to be fairly stable up to 37°C, beyond this the activity got heavily impaired. Further, the GST obtained showed a pH optima of 7.5. Conclusion: Present findings showed that GST from Gc could be conveniently purified using gel filtration chromatography. The purified enzyme showed maximum stability and activity at 4°C.

Item Type: Article
Uncontrolled Keywords: Bubalus bubalis; Gastrothylax crumenifer; Glutathione S-transferase; Purification; Somatic tissue
Subjects: Q Science > QL Zoology
Divisions: Faculties > Faculty of Life Sciences > Department of Zoology
Depositing User: AMU Library
Date Deposited: 29 Jan 2018 09:24
Last Modified: 01 Feb 2018 04:28
URI: http://ir.amu.ac.in/id/eprint/11016

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