Deciphering the toxic effects of organochlorine pesticide, dicofol on human RBCs and lymphocytes

Ahmad, A. and Ahmad, M. (2017) Deciphering the toxic effects of organochlorine pesticide, dicofol on human RBCs and lymphocytes. Pesticide Biochemistry and Physiology, 143. pp. 127-134. ISSN 483575

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Organochlorine pesticides have generated growing concern owing to their diverse toxicities. In this connection, we have evaluated toxic potential of an acaricide, dicofol (DCF) and its harmful effects on human RBCs and lymphocytes. DCF caused hemolysis and rupture of human erythrocytes as confirmed by scanning electron microscopy (SEM). Significant increase in protein oxidation, lipid peroxidation, ROS production, methemoglobin formation with enhanced activities of superoxide dismutase and catalase but decreased level of reduced glutathione were observed as a result of DCF exposure to human erythrocytes. SEM showed significant DCF induced alterations in RBCs from normal discoid shape to echinocytes. Similarly, lymphocytes showed membrane damage, formation of membrane blebs and distorted cell morphology. In vitro comet assay indicated a significant DNA fragmentation in human lymphocytes upon DCF exposure. These results strongly suggest that DCF induces oxidative stress in RBCs via generation of ROS and alters the cellular architecture directly and indirectly.

Item Type: Article
Uncontrolled Keywords: Comet assay; Dicofol; Genotoxicity; Hemolysis; SEM
Divisions: Faculties > Faculty of Life Sciences > Department of Biochemistry
Depositing User: AMU Library
Date Deposited: 03 Feb 2018 07:17
Last Modified: 05 Feb 2018 04:32

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