Alginate encapsulation in Glycyrrhiza glabra L. with phyto-chemical profiling of root extracts of in vitro converted plants using GC-MS analysis

Akhtar, R. and Shahzad, A. (2017) Alginate encapsulation in Glycyrrhiza glabra L. with phyto-chemical profiling of root extracts of in vitro converted plants using GC-MS analysis. Asian Pacific Journal of Tropical Biomedicine, 7 (10). pp. 855-861. ISSN 22211691

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Abstract

Objective To investigate the conversion potential of alginate encapsulated nodes of Glycyrrhiza glabra with phyto-chemical evaluation of root extract of field transferred plants. Methods The excised axenic nodal segments were encapsulated in alginate matrix planted on Murashige and Skoog (1962) medium with different supplementation and formulations of PGRs. The two year old field transferred plants were evaluated for phyto-compounds analysis using GC-MS technique. Results Varied responses were observed during the study, maximum conversion 95.83% ± 2.40% was obtained in these encapsulates when planted on MS medium containing 2.5 μM Kinetin and 0.5 μM α-Naphthalene acetic acid, which eventually developed into complete plantlets in a single step. Further, GC-MS analysis showed the presence of different phyto-compounds in the methanolic root extracts of in vitro converted plants. The results obtained revealed the presence of about 47 phyto-compounds along with various potential bioactive compounds useful for industrial and pharmaceutical purposes. Conclusions This study investigates high frequency regeneration and conversion of Glycyrrhiza glabra in a single step in short time. Also, the in vitro raised plants are analysed for bioactive compounds after field transfer, which shows the presence of numerous compounds useful for commercial and pharmacological purposes.

Item Type: Article
Uncontrolled Keywords: Chemopreventive; Conservation; Licorice; Morphogenic; Synseeds
Subjects: Q Science > QK Botany
Divisions: Faculties > Faculty of Life Sciences > Department of Botany
Depositing User: AMU Library
Date Deposited: 06 Feb 2018 04:18
Last Modified: 06 Feb 2018 04:46
URI: http://ir.amu.ac.in/id/eprint/11117

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