Elucidating the interaction of clofazimine with bovine liver catalase; a comprehensive spectroscopic and molecular docking approach

Zaman, M. and Nusrat, S. and Zakariya, S.M. and Khan, M.V. and Ajmal, M.R. and Khan, R.H. (2017) Elucidating the interaction of clofazimine with bovine liver catalase; a comprehensive spectroscopic and molecular docking approach. Journal of Molecular Recognition, 30 (8). ISSN 9523499

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jmr.261...

Abstract

Nowadays, understanding of interface between protein and drugs has become an active research area of interest. These types of interactions provide structural guidelines in drug design with greater clinical efficacy. Thus, structural changes in catalase induced by clofazimine were monitored by various biophysical techniques including UV-visible spectrometer, fluorescence spectroscopy, circular dichroism, and dynamic light scattering techniques. Increase in absorption spectra (UV-visible spectrum) confers the complex formation between drug and protein. Fluorescence quenching with a binding constants of 2.47 × 104 M−1 revealed that clofazimine binds with protein. Using fluorescence resonance energy transfer, the distance (r) between the protein (donor) and drug (acceptor) was found to be 2.89 nm. Negative Gibbs free energy change (ΔG°) revealed that binding process is spontaneous. In addition, an increase in α-helicity was observed by far-UV circular dichroism spectra by adding clofazimine to protein. Dynamic light scattering results indicate that topology of bovine liver catalase was slightly altered in the presence of clofazimine. Hydrophobic interactions are the main forces between clofazimine and catalase interaction as depicted by molecular docking studies. Apart from hydrophobic interactions, some hydrogen bonding was also observed during docking method. The results obtained from the present study may establish abundant in optimizing the properties of ligand-protein mixtures relevant for numerous formulations. Copyright

Item Type: Article
Uncontrolled Keywords: binding; bovine catalase; clofazimine; hydrogen bonding; hydrophobic interactions; molecular docking
Divisions: Centres > Interdisciplinary Biotechnology Unit
Depositing User: AMU Library
Date Deposited: 07 Feb 2018 05:52
Last Modified: 07 Feb 2018 06:04
URI: http://ir.amu.ac.in/id/eprint/11173

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