Identification and expression analysis of alternatively spliced new transcript isoform of Bax gene in mouse

Husain, M.A. and Ishqi, H.M. and Sarwar, T. and Rehman, S.U. and Tabish, M. (2017) Identification and expression analysis of alternatively spliced new transcript isoform of Bax gene in mouse. Gene, 621. pp. 21-31. ISSN 3781119

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Abstract

Bax, a pro-apoptotic member of Bcl-2 family regulates apoptosis through homodimerization/heterodimerization with Bcl-2. Bax-α is the only product of the Bax gene that has been extensively studied. Bax-α exists in inactive form and several conformational changes are required during apoptosis to activate it. Here, we have identified a novel transcript variant of Bax gene in mouse which contains alternatively spliced new first exon that is different from the first exon of previously reported transcript. Conceptual translation of new transcript encodes a protein (Bax-α1), having different N-terminus. The existence of the new transcript variant was confirmed by reverse transcriptase-PCR, semi-nested PCR using primers designed for the newly identified transcript variant. The identity of PCR product obtained after semi-nested PCR was confirmed by DNA sequencing. Relative expression of new transcript variant with respect to reported transcript was also studied with the help of real time PCR. The existence of new transcript variant was further supported by the presence of clusters of overlapping ESTs from the database. Bax-α1 possibly displays heterogeneous properties as predicted by post-translational modification analysis tools. The differences in post-translational modifications might play important roles in divergent function of the new isoform. The three dimensional structure was generated by homology modelling to visualize the differences at N termini of known and newly identified variant.

Item Type: Article
Uncontrolled Keywords: Bax; Homology modelling; RT PCR; Semi-nested PCR; Transcript variant
Subjects: Q Science > Q Science (General)
Divisions: Faculties > Faculty of Life Sciences > Department of Biochemistry
Depositing User: AMU Library
Date Deposited: 07 Feb 2018 07:54
Last Modified: 07 Feb 2018 10:29
URI: http://ir.amu.ac.in/id/eprint/11187

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