Estimation of genetic variability, mutagenic effectiveness and efficiency in M2 flower mutant lines of Capsicum annuum L. treated with caffeine and their analysis through RAPD markers

Aslam, R. and Bhat, T.M. and Choudhary, S. and Ansari, M.Y.K. and Shahwar, D. (2017) Estimation of genetic variability, mutagenic effectiveness and efficiency in M2 flower mutant lines of Capsicum annuum L. treated with caffeine and their analysis through RAPD markers. Journal of King Saud University - Science, 29 (3). pp. 274-283. ISSN 10183647

Full text not available from this repository.
Official URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

In the present investigation healthy and certified seeds of Capsicum annuum were treated with five concentrations of caffeine i.e. 0.10%, 0.25%, 0.50%, 0.75% and 1.0%. Germination percentage, plants survival and pollen fertility were decreased with the increase of caffeine concentrations. Similarly root length and shoot length were decreased as the concentrations increased in M1 generation. Different mutants were isolated in M1 generation. In M2 generation, various flower mutants with changes in number of sepals, petals, anther size colour i.e. Trimerous, tetramerous, pentamerous with fused petals, hexamerous etc were segregated. Heptamerous and anther change was not observed in lower concentration viz. 0.1%. All these mutants showed significant changes in morphological characters and good breeding values at lower and intermediate concentrations. Mutagenic effectiveness and efficiency was observed on the basis of M2 flower mutant frequency. It was generally decreased with the increase of mutagen concentrations. Cytological aberrations in mutants showed the decreasing trend at meiotic final stages. These mutants were further analysed through RAPD method and on the basis of appearance of polymorphic DNA bands, they distinguished these flower mutants genotypically. Among 93 bands 44 bands were polymorphic which showed great genetic variation produced by caffeine. As an outcome of that the above caffeine concentrations are good for the induction of genetic variability in Capsicum genotype.

Item Type: Article
Uncontrolled Keywords: Caffeine; Capsicum; Genetic variability; Mutagenic effectiveness and efficiency; RAPD
Subjects: Q Science > QK Botany
Divisions: Faculties > Faculty of Life Sciences > Department of Botany
Depositing User: AMU Library
Date Deposited: 07 Feb 2018 09:32
Last Modified: 07 Feb 2018 10:32
URI: http://ir.amu.ac.in/id/eprint/11194

Actions (login required)

View Item View Item