Ellagic acid mitigates arsenic-trioxide-induced mitochondrial dysfunction and cytotoxicity in SH-SY5Y cells

Firdaus, F. and Zafeer, M.F. and Waseem, M. and Anis, E. and Hossain, M.M. and Afzal, M. (2018) Ellagic acid mitigates arsenic-trioxide-induced mitochondrial dysfunction and cytotoxicity in SH-SY5Y cells. Journal of Biochemical and Molecular Toxicology, 32 (2). ISSN 10956670

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Official URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/jb...

Abstract

In the current study, neuroprotective significance of ellagic acid (EA, a polyohenol) was explored by primarily studying its antioxidant and antiapoptotic potential against arsenic trioxide (As2O3)-induced toxicity in SH-SY5Y human neuroblastoma cell lines. The mitigatory effects of EA with particular reference to cell viability and cytotoxicity, the generation of reactive oxygen species, DNA damage, and mitochondrial dynamics were studied. Pretreatment of SH-SY5Y cells with EA (10 and 20 μM) for 60 min followed by exposure to 2 μM As2O3 protected the SH-SY5Y cells against the harmful effects of the second. Also, EA pre-treated groups expressed improved viability, repaired DNA, reduced free radical generation, and maintained altered mitochondrial membrane potential than those exposed to As2O3 alone. EA supplementation also inhibited As2O3-induced cytochrome c expression that is an important hallmark for determining mitochondrial dynamics. Thus, the current investigations are more convinced for EA as a promising candidate in modulating As2O3-induced mitochondria-mediated neuronal toxicity under in vitro system.

Item Type: Article
Uncontrolled Keywords: Apoptosis; Ellagic acid; Flow cytometry; Membrane potential; Mitochondria
Subjects: Q Science > QL Zoology
R Medicine > RZ Other systems of medicine
Divisions: Centres > Interdisciplinary Brain Research Center
Centres > Interdisciplinary Brain Research Center
Depositing User: AMU Library
Date Deposited: 24 Mar 2018 04:50
Last Modified: 24 Mar 2018 04:50
URI: http://ir.amu.ac.in/id/eprint/11279

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