Unsaturated aldehyde, 4-hydroxynonenal (HNE) alters the structural integrity of HSA with consequences in the immuno-pathology of rheumatoid arthritis

Khan, F. and Moinuddin, . and Mir, A.R. and Islam, S. and Abidi, M. and Husain, M.A. and Khan, R.H. (2018) Unsaturated aldehyde, 4-hydroxynonenal (HNE) alters the structural integrity of HSA with consequences in the immuno-pathology of rheumatoid arthritis. International Journal of Biological Macromolecules, 112. pp. 306-314. ISSN 01418130

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Abstract

Human serum albumin (HSA) - the most abundant plasma protein plays an important role in the transport of endogenous and exogenous molecules in the body. Its modifications have been implicated in a variety of pathological disorders. We have studied the interaction of HNE with HSA at a molecular level by docking experiment and the results suggest a strong interaction between HNE and HSA. Immunological studies revealed that the circulating auto-antibodies in rheumatoid arthritis (RA) patients have a stronger affinity towards HNE-modified HSA. The HSA isolated from RA patients (RA-HSA) exhibited HNE mediated damage in its secondary and tertiary structure when compared to HSA derived from healthy human subjects (NH-HSA). RA patients presented a significant rise in carbonyls and a considerable decline in free thiol content. Preferential binding of experimentally induced anti-HNE-HSA antibodies to RA-HSA over NH-HSA was observed by ELISA. The results suggest HNE induced structural perturbations in HSA with neoepitopes that generate anti-HNE-HSA antibodies in RA. Hence, HNE-HSA may provide lead towards the development of a biomarker for the disease.

Item Type: Article
Uncontrolled Keywords: Reactive carbonyl species; Rheumatoid arthritis; α,β‑Unsaturated aldehydes
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculties > Faculty of Medicine > J.N. Medical College > Department of Biochemistry
Depositing User: AMU Library
Date Deposited: 24 Mar 2018 06:13
Last Modified: 24 Mar 2018 06:13
URI: http://ir.amu.ac.in/id/eprint/11284

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