Acetaldehyde-induced structural and conformational alterations in human immunoglobulin G: A physicochemical and multi-spectroscopic study

Waris, S. and Habib, S. and Tantry, I.Q. and Khan, R.H. and Mahmood, R. and Ali, A. (2018) Acetaldehyde-induced structural and conformational alterations in human immunoglobulin G: A physicochemical and multi-spectroscopic study. International Journal of Biological Macromolecules, 113. pp. 701-710. ISSN 01418130

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Abstract

Acetaldehyde is a reactive aldehyde produced as an intermediate of alcohol metabolism and tobacco pyrolysis. It has the potential to interact with different biomolecules in various tissues which results in the formation of stable, unstable and covalent adducts. This causes structural and functional modifications that may lead to severe complications such as cancer. This study has probed the structural modifications in human immunoglobulin G (IgG) as a function of different concentrations of acetaldehyde in the presence of reducing agent, sodium borohydride. Acetaldehyde mediated modifications in IgG have been characterised by various physicochemical techniques. UV-spectrophotometry showed that acetaldehyde modified IgG exhibited marked increase in hyperchromicity. Fluorescence studies revealed a significant quenching of tryptophan fluorescence which resulted in loss of β-sheet secondary structure that was confirmed by circular dichroic analysis. Gross structural changes in the morphology of IgG were confirmed by increase in mass and hydrodynamic radius of this glycoprotein along with the appearance of fibrillar structures in modified IgG, when compared to the granular structure of the native form of IgG observed by scanning electron microscope. The results indicate that acetaldehyde causes alterations in the secondary and tertiary structure of the protein leading to diminution of normal function of IgG molecule.

Item Type: Article
Uncontrolled Keywords: Acetaldehyde; Human IgG; Spectroscopy
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculties > Faculty of Medicine > J.N. Medical College > Department of Biochemistry
Depositing User: AMU Library
Date Deposited: 24 Mar 2018 10:57
Last Modified: 24 Mar 2018 10:57
URI: http://ir.amu.ac.in/id/eprint/11291

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