Utility of cytospin and cell block technology in evaluation of body fluids and urine samples: A comparative study

Qamar, I. and Rehman, S. and Mehdi, G. and Maheshwari, V. and Ansari, H.A. and Chauhan, S. (2018) Utility of cytospin and cell block technology in evaluation of body fluids and urine samples: A comparative study. Journal of Cytology, 35 (2). pp. 79-82. ISSN 09709371

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Official URL: http://www.jcytol.org/article.asp?issn=0970-9371;y...


Background: Cytologic examination of body fluids commonly involves the use of direct or sediment smears, cytocentrifuge preparations, membrane filter preparations, or cell block sections. Cytospin and cell block techniques are extremely useful in improving cell yield of thin serous effusions and urine samples, and ensure high diagnostic efficacy. Materials and Methods: We studied cytospin preparations and cell block sections prepared from 180 samples of body fluids and urine samples to compare the relative efficiency of cell retrieval, preservation of cell morphology, ease of application of special stains, and diagnostic efficacy. Samples were collected and processed to prepare cytospin smears and cell block sections. Results: We observed that overall, cell yield and preservation of individual cell morphology were better in cytospin preparations as compared to cell blocks, while preservation of architectural pattern was better in cell block sections. The number of suspicious cases also decreased on cell block sections, with increased detection of malignancy. It was difficult to prepare cell blocks from urine samples due to low cellularity. Conclusions: Cytospin technology is a quick, efficient, and cost-effective method of increasing cell yield in hypocellular samples, with better preservation of cell morphology. Cell blocks are better prepared from high cellularity fluids; however, tissue architecture is better studied, with improved rate of diagnosis and decrease in ambiguous results. Numerous sections can be prepared from a small amount of material. Special stains and immunochemical stains can be easily applied to cell blocks. It also provides a source of archival material.

Item Type: Article
Uncontrolled Keywords: Body fluids; Cell block; Cytospin; Urine sample
Subjects: R Medicine > RB Pathology
Divisions: Faculties > Faculty of Medicine > J.N. Medical College
Depositing User: AMU Library
Date Deposited: 07 Apr 2018 04:51
Last Modified: 07 Apr 2018 04:51
URI: http://ir.amu.ac.in/id/eprint/11324

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